The mother of Madeleine McCann was close to tears yesterday as she dismissed suggestions that her daughter was killed in her bedroom on the night that she disappeared from the family’s holiday apartment.

Kate McCann and her husband, Gerry, are expected to be interviewed again by detectives after reports that blood had been found in their daughter’s bedroom and evidence that it had contained a body.

The focus of the police investigation has shifted to the couple and the British friends who were with them in the Portuguese resort of Praia da Luz.

Police sources are reported to have abandoned the theory that Madeleine has been abducted and may still be alive. Officers are now convinced that Madeleine died in her bedroom as a result of “negligence or murder”, claimed the Diário de Notícias newspaper. However, the McCanns insisted yesterday that they still believed that Madeleine was alive when she was taken from the apartment 97 days ago.

Mrs McCann, 38, said: “Last week when we met with the police they said, ‘We are looking for a living child’. And they’ve said that a lot.”

The couple refused to discuss reports that a speck of blood had been found by a British sniffer dog in the bedroom where Madeleine was sleeping, although Mrs McCann was visibly upset when questioned. The blood sample will be sent for DNA analysis to see if it matches the girl or a kidnapper. However, it may be too small to carry out the test.

Mr McCann said: “Kate and I strongly believe Madeleine was alive when she was taken from the apartment. Obviously what we don’t know is what happened to her afterwards, who’s taken her and what the motive is.

“We’re not naive, but on numerous occasions the Portuguese police have said they are looking for Madeleine alive and not Madeleine being murdered. I don’t know of any information to have changed that.”

The couple are to be shown the photograph of a child snatcher who was in the area of Praia da Luz when their daughter disappeared. Urs Von Aesch, 67, killed himself last week while being hunted after abducting Ylenia Lenhard, a five-year-old Swiss girl who looks like Madeleine.

Officers in St Gallen, central Switzerland, close to where Ylenia disappeared, have also reopened files on five other girls who disappeared in the area before von Aesch moved to Benimantell, near the Spanish coast. A Swiss citizen, he was jailed in the 1960s after trying to blackmail a businessman by threatening to kidnap his young son. He went on to become a successful businessman before moving to Spain with his Spanish wife in about 1990.

Mr and Mrs McCann, from Rothley, Leicestershire, will be shown his photograph while they are questioned separately at the headquarters of the Polícia Judiciária in Portimao as part of a review of the entire investigation. They have already been interviewed twice at the headquarters.

A Renault car that had been used by the couple and their friends after Madeleine disappeared was still being investigated yesterday by police officers and forensic scientists. Tests have also been carried out on a car belonging to Robert Murat, the only official suspect in the case. The cars of his mother, his girlfriend, her estranged husband and Sergei Malinka, a web designer who had worked for Mr Murat, have also been tested.

Mr McCann said that he and his wife expected to be treated with the same thoroughness as anyone else in the investigation. “We wouldn’t expect it any other way,” he said. “The same high levels will be applied to us as would be applied to anybody else, and that’s only right and proper.”

Madeleine was not seen alive by anyone apart from her parents for almost four hours on the night that she disappeared while sleeping in a room with the McCanns’ two-year-old twins, Amelie and Sean, it was claimed yesterday. She was put to bed at 6pm and Mr McCann said that he saw her an hour before his wife reported her missing at 10pm. Other friends in the group had checked on the apartment but had not entered the bedroom.

Portuguese police are reported to be concerned about inconsistencies between the statements given by the McCanns and their friends, three other couples and a single woman. The couples are Russell O’Brien and Jane Tanner, both 36, from Exeter, Fiona Payne, 34, and her husband, David, from Leicester, and Rachel Oldfield, 36, and her husband, Matthew, from London.

British and Portuguese police have been monitoring at least one of the friends and a worker connected to the Ocean Club resort, where the group stayed, although police have previously said that none of the group was a suspect.

A source close to the investigation said that the McCanns and their friends would be interviewed as part a routine review of the investigation being carried out with the help of British police officers. He said that possible traces of blood in Madeleine’s ground-floor bedroom could have come from any of the hundreds of people who had stayed in the apartment in recent years. Experts also cast doubt on claims that a British dog trained to track bodies had detected a scent in the bedroom. The body would have had to be in the room for at least several hours before leaving a detectable trace.

Mr Murat has already been reinterviewed and a search at the weekend of his home and garden using British dogs and sonar equipment failed to reveal any trace of Madeleine.

Mr Murat, who previously lived in Hockering, Norfolk, with his wife and young daughter, denies any involvement in the kidnapping.

The missing hours

April 28 McCanns arrive in Praia da Luz with Madeleine, 3, and Sean and Amelie, 2.

May 3, 6pm McCanns’ three children are put to bed. Madeleine says it has been “the best day ever”

7pm Mr and Mrs McCann leave for meal with friends at tapas restaurant less than 50 metres from apartment

9pm Mr McCann checks the children. He is believed to be the last person known to have seen Madeleine

9-10pm Friends check on each others’ children. No one entered Madeleine’s room, to avoid waking children

9.30pm Jane Tanner sees man carrying child close to McCanns’ apartment. She does not think it suspicious

10pm Kate McCann discovers Madeleine missing

10.05pm Guests and staff search Ocean Club resort until 4.30am

10.15pm Police are called

May 4, 8am Hunt for Madeleine becomes full-scale child abduction investigation. Border police, Spanish authorities and airports notified